ABSTRACT
Clove oil inhibited human platelet aggregation induced by arachidonic acid [AA], platelet-activating factor [PAF] or collagen. Clove oil was a more effective inhibitor for aggregation induced by AA and PAF [IC[50]: 4 and 6 micro M respectively] than collagen [IC[50] : 132 micro M]. The in vivo experiments in rabbits showed that clove oil [50-100mg/kg] afforded 100% protection against PAF [11mg/kg, i.v.] and 70% protection against AA [2.0 mg/kg, i.v.]- induced thrombosis and shock due to pulmonary platelet thrombosis. It also inhibited thromboxane-A[2] and 12-HETE production by human platelets incubated with [C[14]] AA. These results are indicative that clove oil is inhibitory of platelet aggregation and thromboxane synthesis and may act as anti- thrombotic agent